Biological Nitrogen Removal Database

Zobellella taiwanensis strain ZT1

• Phylum : Proteobacteria
• Class : Gammaproteobacteria
• Order :Aeromonadales
• Family : Alteromonadaceae
• Genus : Zobellella

NO₃^-

Glucose, Sucrose or Mannitol

Reference:Lin and Shieh., 2006

Title:Zobellella Denitrificans Gen. Nov., Sp. Nov. And Zobellella Taiwanensis Sp. Nov., Denitrifying Bacteria Capable of Fermentative Metabolism

Pubmed ID:16738093

Pubmed link:Link

Full research link:Link

Abstract: Two denitrifying strains of heterotrophic, facultatively anaerobic bacteria, designated ZD1T and ZT1T, were isolated from sediment samples collected from mangrove ecosystems in Taiwan. The isolates were Gram-negative. Cells grown in broth cultures were straight rods that were motile by means of a single polar flagellum. The isolates grew optimally in 1–3 % NaCl, but NaCl was not an absolute requirement for growth; only strain ZT1T grew in 13–14 % NaCl. Both isolates grew between 10 and 45 °C, with optimum growth at 30–35 °C. They were capable of anaerobic growth by denitrifying metabolism using nitrate or nitrous oxide as terminal electron acceptors or, alternatively, by fermenting glucose, sucrose or mannitol as substrates. C18 : 1 ω7c was the most abundant fatty acid (32.6–35.7 %). The other major fatty acids included C16 : 1 ω7c (27.5–29.4 %) and C16 : 0 (20.1–22.0 %). The two isolates had 16S rRNA gene sequence similarity of 96.8 % and shared 94.1–96.8 % sequence similarity with the most closely related species, Oceanimonas doudoroffii, Oceanimonas baumannii, Oceanimonas smirnovii and Oceanisphaera litoralis. They could be distinguished from these species in that they were capable of fermentative metabolism, had relatively high DNA G+C contents (62.0–64.0 mol%) and contained C18 : 1 ω7c instead of C16 : 1 ω7c as the most abundant fatty acid. Characterization data accumulated in this study revealed that the two denitrifying isolates could be classified as representatives of two novel species in a new genus, Zobellella gen. nov., with Zobellella denitrificans sp. nov. (type strain ZD1T=BCRC 17493T=JCM 13380T) as the type species and Zobellella taiwanensis sp. nov. (type strain ZT1T=BCRC 17494T=JCM 13381T) as a second species.